Recombinant CD35 antibody

Catalog No. ABIN7072443

This anti-CD35 antibody is a Rabbit Chimeric antibody detecting CD35 in FACS, IF, ELISA, IA and in vivo. Suitable for Human.

Quick Overview for Recombinant CD35 antibody (ABIN7072443)

Target: CD35 (CR1) (Complement Component Receptor 1 (CD35) (CR1))

Antibody Type: Recombinant Antibody

Reactivity: Human

Host: Rabbit

Clonality: Chimeric

Conjugate: This CD35 antibody is un-conjugated

Application: Flow Cytometry (FACS), Immunofluorescence (IF), ELISA, Immunoassay (IA), In vivo Studies (in vivo)

Clone: HB-8592

Product Details anti-CD35 Antibody

Purpose: Anti-CD35 [HB-8592], Rabbit IgG, kappa

Specificity: This antibody is specific for Human CR1 but does not compete with clones 7G9 or E11, it binds to a site distinct from the C3b binding site and can be used to measure the number of receptors despite occupancy by ligand. This antibody cross-reacts with Chimpanzee, Baboon, Rhesus, and Cynomolgus monkey CR1. The scFv version of this antibody reacted with Mouse CR1.

Cross-Reactivity: Baboon, Chimpanzee, Cynomolgus, Mouse, Rhesus Monkey

Characteristics:

Original Species of Ab: Mouse

Original Format of Ab: IgG1

Purification: Protein A affinity purified

Immunogen: The original antibody was generated by immunizing mice with purified CR1 from human erythrocytes.

Isotype: IgG kappa

Application Details

Application Notes: This antibody has been used for the analysis of the expression of the CR1 receptor on macrophages isolated from the peritoneal effluents of CAPD patients with peritonitis and human leukemia-derived CD4⁺ T cell lines by flow cytometry (Hart et al., 1992, PMID: 1606734) (Yaskanin and Waxman, 1995, PMID: 7758124). This antibody was used in immune adherence (IA) binding and release assays (Edberg et al., 1992, PMID: 1376254). This antibody was used in in vivo clearance experiments (Taylor et al., 1997, PMID: 9000042). An scFv version of this antibody was generated, its pharmacokinetics were evaluated, and it was shown to bind mouse mixed lymphocytes and bone marrow-derived dendritic cells in an ELISA and to bind CR1-expressing mouse bone marrow-derived dendritic cells via immunofluorescence labeling (Knight et al., 2008, PMID: 18385459).

Comment:

This chimeric rabbit antibody was made using the variable domain sequences of the original Mouse IgG1 format, for improved compatibility with existing reagents, assays and techniques.

Restrictions: For Research Use only

Handling

Concentration: 1 mg/mL

Buffer: PBS with 0.02 % Proclin 300.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C.

Target Details for CD35

Target: CD35 (CR1) (Complement Component Receptor 1 (CD35) (CR1))

Alternative Name: CD35

Background: CR1, C3BR, C4BR, HB8592, HB 8592, MoAb 543

UniProt: P17927

Pathways: Complement System, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Endopeptidase Activity